Characterization of a membrane-associated, receptor and G-protein responsive Phosphoinositide-specific Phospholipase C from avian erythrocytes
- Ruiz-Larrea, F. 2
- Berrie, C.P. 1
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1
University of Liverpool
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2
Centro Nacional de Biotecnología
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ISSN: 0014-5793
Año de publicación: 1993
Volumen: 328
Número: 1-2
Páginas: 174-182
Tipo: Artículo
Otras publicaciones en: FEBS Letters
Resumen
We describe the reconstitution and purification of a membrane-associated phosphoinositide-specific phospholipase C (PIC) from turkey erythrocyte ghosts. This PIC is responsive to a G-protein coupled to P(2y) purinergic receptors which are expressed in turkey erythrocytes. Reconstitution is achieved by adding partially purified PIC to [ 3H]inositol-prelabelled turkey erythrocyte membranes depleted of their endogenous PIC (acceptor membranes). PIC activity is associated with a 52 kDa polypeptide on SDS-polyacrylamide gel electrophoresis. Addition of a 307-fold purified enzyme to the acceptor membranes has no effect on basal PIC activity, but markedly increases the response to GTPγS and P(2y)-purinergic receptor activation.