Characterization of a membrane-associated, receptor and G-protein responsive Phosphoinositide-specific Phospholipase C from avian erythrocytes

  1. Ruiz-Larrea, F. 2
  2. Berrie, C.P. 1
  1. 1 University of Liverpool
    info

    University of Liverpool

    Liverpool, Reino Unido

    ROR https://ror.org/04xs57h96

  2. 2 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

Zeitschrift:
FEBS Letters

ISSN: 0014-5793

Datum der Publikation: 1993

Ausgabe: 328

Nummer: 1-2

Seiten: 174-182

Art: Artikel

DOI: 10.1016/0014-5793(93)80988-7 PMID: 8393807 SCOPUS: 2-s2.0-0027293268 GOOGLE SCHOLAR

Andere Publikationen in: FEBS Letters

Institutionelles Repository: lock_openOpen Access Editor

Zusammenfassung

We describe the reconstitution and purification of a membrane-associated phosphoinositide-specific phospholipase C (PIC) from turkey erythrocyte ghosts. This PIC is responsive to a G-protein coupled to P(2y) purinergic receptors which are expressed in turkey erythrocytes. Reconstitution is achieved by adding partially purified PIC to [ 3H]inositol-prelabelled turkey erythrocyte membranes depleted of their endogenous PIC (acceptor membranes). PIC activity is associated with a 52 kDa polypeptide on SDS-polyacrylamide gel electrophoresis. Addition of a 307-fold purified enzyme to the acceptor membranes has no effect on basal PIC activity, but markedly increases the response to GTPγS and P(2y)-purinergic receptor activation.