Characterization of a membrane-associated, receptor and G-protein responsive Phosphoinositide-specific Phospholipase C from avian erythrocytes
- Ruiz-Larrea, F. 2
- Berrie, C.P. 1
-
1
University of Liverpool
info
-
2
Centro Nacional de Biotecnología
info
ISSN: 0014-5793
Year of publication: 1993
Volume: 328
Issue: 1-2
Pages: 174-182
Type: Article
More publications in: FEBS Letters
Abstract
We describe the reconstitution and purification of a membrane-associated phosphoinositide-specific phospholipase C (PIC) from turkey erythrocyte ghosts. This PIC is responsive to a G-protein coupled to P(2y) purinergic receptors which are expressed in turkey erythrocytes. Reconstitution is achieved by adding partially purified PIC to [ 3H]inositol-prelabelled turkey erythrocyte membranes depleted of their endogenous PIC (acceptor membranes). PIC activity is associated with a 52 kDa polypeptide on SDS-polyacrylamide gel electrophoresis. Addition of a 307-fold purified enzyme to the acceptor membranes has no effect on basal PIC activity, but markedly increases the response to GTPγS and P(2y)-purinergic receptor activation.