Molecular characterization of antibiotic resistance in Escherichia coli strains from a dairy cattle farm and its surroundings

  1. Navajas-Benito, E.V. 1
  2. Alonso, C.A. 1
  3. Sanz, S. 1
  4. Olarte, C. 1
  5. Martínez-Olarte, R. 1
  6. Hidalgo-Sanz, S. 2
  7. Somalo, S. 1
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 CINFA, Pamplona, Spain
Revista:
Journal of the Science of Food and Agriculture

ISSN: 0022-5142

Año de publicación: 2017

Volumen: 97

Número: 1

Páginas: 362-365

Tipo: Artículo

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DOI: 10.1002/JSFA.7709 SCOPUS: 2-s2.0-84963548279 WoS: WOS:000387349900047 GOOGLE SCHOLAR

Otras publicaciones en: Journal of the Science of Food and Agriculture

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Resumen

BACKGROUND: This study describes the phenotypic and genotypic characteristics of 78 genetically different Escherichia coli recovered from air and exudate samples of a dairy cattle farm and its surroundings in Spain, in order to gain insight into the flow of antimicrobial resistance through the environment and food supply. RESULTS: Antimicrobial resistance was detected in 21.8% of the 78 E. coli isolates analyzed (resistance for at least one of the 14 agents tested). The highest resistance rates were recorded for ampicillin, nalidixic acid, trimethoprim/sulfamethoxazole and tetracycline. The resistance genes detected were as follows (antibiotic (number of resistant strains), gene (number of strains)): ampicillin (9), blaTEM-1 (6); tetracycline (15), tet(A) (7), tet(B) (4), tet(A) + tet(B) (1); chloramphenicol (5), cmlA (2), floR (2); trimethoprim/sulfamethoxazole (10), sul2 (4), sul1 (3), sul3 (2), sul1 + sul2 (1); gentamicin-tobramycin (1), ant(2″) (1). About 14% of strains showed a multidrug-resistant phenotype and, of them, seven strains carried class 1 integrons containing predominantly the dfrA1-aadA1 array. One multidrug-resistant strain was found in both inside and outside air, suggesting that the airborne spread of multidrug-resistant bacteria from the animal housing facilities to the surroundings is feasible. CONCLUSIONS: This study gives a genetic background of the antimicrobial resistance problem in a dairy cattle farm and shows that air can act as a source for dissemination of antimicrobial-resistant bacteria. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry