Development of a liquid-liquid extraction method for the determination of pyrimethanil, metalaxyl, dichlofluanid and penconazol during the fermentative process of must by GC-NPD

  1. Vaquero-Fernández, L. 1
  2. Sanz-Asensio, J. 1
  3. Fernández-Zurbano, P. 12
  4. Sainz-Ramírez, M. 1
  5. López-Alonso, M. 1
  6. Epifanio-Fernández, S.-I. 1
  7. Martínez-Soria, M.-T. 1
  1. 1 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España


  2. 2 Instituto de Ciencias de la Vid y del Vino

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España


Journal of the Science of Food and Agriculture

ISSN: 0022-5142

Year of publication: 2009

Volume: 89

Issue: 5

Pages: 750-757

Type: Article

DOI: 10.1002/JSFA.3508 SCOPUS: 2-s2.0-63849233493 WoS: WOS:000264758400003 GOOGLE SCHOLAR

More publications in: Journal of the Science of Food and Agriculture

Institutional repository: lockOpen access editor


Cited by

  • Scopus Cited by: 12 (12-01-2023)
  • Web of Science Cited by: 8 (06-02-2023)

JCR (Journal Impact Factor)

  • Year 2009
  • Journal Impact Factor: 1.386
  • Journal Impact Factor without self cites: 1.226
  • Article influence score: 0.433
  • Best Quartile: Q1
  • Area: AGRICULTURE, MULTIDISCIPLINARY Quartile: Q1 Rank in area: 6/45 (Ranking edition: SCIE)
  • Area: FOOD SCIENCE & TECHNOLOGY Quartile: Q2 Rank in area: 39/118 (Ranking edition: SCIE)
  • Area: CHEMISTRY, APPLIED Quartile: Q2 Rank in area: 26/64 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2009
  • SJR Journal Impact: 0.872
  • Best Quartile: Q1
  • Area: Food Science Quartile: Q1 Rank in area: 33/243
  • Area: Agronomy and Crop Science Quartile: Q1 Rank in area: 40/306
  • Area: Nutrition and Dietetics Quartile: Q1 Rank in area: 29/119
  • Area: Biotechnology Quartile: Q2 Rank in area: 74/248


BACKGROUND: A rapid, selective and sensitive gas chromatographic method is described for the analysis of four fungicides - pyrimethanil, metalaxyl, penconazol and dichlofluanid, widely used in vineyards - in must and wine, as well as samples during alcoholic fermentation. The effect of alcoholic fermentation on pyrimethanil concentration in synthetic and real musts was studied. RESULTS: The proposed method was based on liquid-liquid extraction followed by gas chromatography with nitrogen-phosphorus detector. n-Hexane was the most appropriate solvent for extracting the target fungicides in synthetic and real samples. Quality parameters of the proposed method presented good recovery (ca 78.3% for almost all compounds) and precision (between 1.4% and 6.1%), and quantification limits lower than maximum residue limits. However, for dichl of luanid recovery levels of well over 100% were found. CONCLUSIONS: There was no matrix effect in the analysis of pyrimethanil, metalaxyl or penconazol, but this effect was observed for dichlofluanid in synthetic and real matrices. The application of the fermentative process on pyrimethanil fungicide caused a high decrease in the concentration of this compound, and no product appeared in degradation. The decrease of pyrimethanil in fermented samples was similar in both synthetic and real samples. © 2009 Society of Chemical Industry.