Phenotypic and genotypic characterization of bacteriocins in clinical enterococcal isolates of Tunisia

  1. Klibi, N. 3
  2. Jouini, A. 3
  3. Rojo-Bezares, B. 1
  4. Masmoudi, A. 2
  5. Ruiz-Larrea, F. 1
  6. Boudabous, A. 3
  7. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hôpital La Rabta
    info

    Hôpital La Rabta

    Túnez, Túnez

    ROR https://ror.org/00gffbx54

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
World Journal of Microbiology and Biotechnology

ISSN: 0959-3993

Año de publicación: 2008

Volumen: 24

Número: 5

Páginas: 653-657

Tipo: Artículo

DOI: 10.1007/S11274-007-9519-Z SCOPUS: 2-s2.0-42049086105 WoS: WOS:000254805700010 GOOGLE SCHOLAR

Otras publicaciones en: World Journal of Microbiology and Biotechnology

Resumen

The presence of bacteriocin structural genes (entA, entB, entP, entQ, entAS-48, entL50A/B, bac31, and cylL) encoding different bacteriocins (enterocin A, enterocin B, enterocin P, enterocin Q, enterocin AS-48, enterocin L50A/B, bacteriocin 31 and cytolysin L, respectively), and the production of bacteriocin activity were analysed in 139 E. faecalis and 41 E. faecium clinical isolates of Tunisia. Forty-eight of 139 E. faecalis isolates (34%) and 7 of 41 of E. faecium isolates (17%) were bacteriocin producers. Sixty-two per cent of the bacteriocin-producing enterococci showed inhibitory activity against L. monocytogenes. Different combinations of entA, entB, entP, and entL50A/B genes were detected among the seven bacteriocin-producer E. faecium isolates, and more that one gene were identified in all the isolates. The entA gene was associated in most of the cases with entB gene in E. faecium isolates. Cyl LS were the unique genes detected among E. faecalis (in 24 of 48 bacteriocin-producer isolates, 50%). A β-hemolytic activity was demonstrated in 19 of the 24 cyl LS -positive E. faecalis isolates (79%), this activity being negative in the remaining five isolates. The presence of different bacteriocin structural genes and the production of antimicrobial activities seems to be a common trait of clinical enterococci. © 2007 Springer Science+Business Media B.V.