Phenotypic and genotypic characterization of bacteriocins in clinical enterococcal isolates of Tunisia
- Klibi, N. 3
- Jouini, A. 3
- Rojo-Bezares, B. 1
- Masmoudi, A. 2
- Ruiz-Larrea, F. 1
- Boudabous, A. 3
- Torres, C. 1
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1
Universidad de La Rioja
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2
Hôpital La Rabta
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3
Université de Tunis El Manar
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ISSN: 0959-3993
Argitalpen urtea: 2008
Alea: 24
Zenbakia: 5
Orrialdeak: 653-657
Mota: Artikulua
Beste argitalpen batzuk: World Journal of Microbiology and Biotechnology
Laburpena
The presence of bacteriocin structural genes (entA, entB, entP, entQ, entAS-48, entL50A/B, bac31, and cylL) encoding different bacteriocins (enterocin A, enterocin B, enterocin P, enterocin Q, enterocin AS-48, enterocin L50A/B, bacteriocin 31 and cytolysin L, respectively), and the production of bacteriocin activity were analysed in 139 E. faecalis and 41 E. faecium clinical isolates of Tunisia. Forty-eight of 139 E. faecalis isolates (34%) and 7 of 41 of E. faecium isolates (17%) were bacteriocin producers. Sixty-two per cent of the bacteriocin-producing enterococci showed inhibitory activity against L. monocytogenes. Different combinations of entA, entB, entP, and entL50A/B genes were detected among the seven bacteriocin-producer E. faecium isolates, and more that one gene were identified in all the isolates. The entA gene was associated in most of the cases with entB gene in E. faecium isolates. Cyl LS were the unique genes detected among E. faecalis (in 24 of 48 bacteriocin-producer isolates, 50%). A β-hemolytic activity was demonstrated in 19 of the 24 cyl LS -positive E. faecalis isolates (79%), this activity being negative in the remaining five isolates. The presence of different bacteriocin structural genes and the production of antimicrobial activities seems to be a common trait of clinical enterococci. © 2007 Springer Science+Business Media B.V.