Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines

  1. Cauré Barbosa Portugal 1
  2. Fernanda Ruiz Larrea 1
  1. 1 Instituto de Ciencias de la Vid y del Vino
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    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

Revista:
American Journal of Enology and Viticulture

ISSN: 0002-9254

Año de publicación: 2013

Volumen: 64

Número: 1

Páginas: 139-145

Tipo: Artículo

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DOI: 10.5344/AJEV.2012.12047 DIALNET GOOGLE SCHOLAR

Otras publicaciones en: American Journal of Enology and Viticulture

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2011/00005/001

Resumen

Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.

Fuente de los datos: Dialnet