Development and characterization of new microsatellite markers for grape

  1. Arroyo-García, R. 12
  2. Martínez-Zapater, J.M. 12
  1. 1 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  2. 2 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

Revue:
Vitis

ISSN: 0042-7500

Année de publication: 2004

Volumen: 43

Número: 4

Pages: 175-178

Type: Article

D'autres publications dans: Vitis

Dépôt institutionnel: lock_openAccès ouvert Editor

Résumé

Thirty five new grape microsatellite markers were developed under an international consortium involving AGROGENE. These loci were amplified in 41 Spanish cultivars of V. vinifera. Eleven of the markers were polymorphic and informative in V. vinifera. Twelve were monomorphic and of the remaining markers one was polymorphic but less useful because individuals amplified more than two bands and the rest had amplification problems. The number of alleles detected for the 11 informative markers ranged from 4 to 12, with heterozygosity values ranging from 0.6 to 0.8. Primer sequences are reported for these markers.