Development and characterization of new microsatellite markers for grape
- Arroyo-García, R. 12
- Martínez-Zapater, J.M. 12
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1
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
Madrid, España
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2
Centro Nacional de Biotecnología
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ISSN: 0042-7500
Any de publicació: 2004
Volum: 43
Número: 4
Pàgines: 175-178
Tipus: Article
Altres publicacions en: Vitis
Resum
Thirty five new grape microsatellite markers were developed under an international consortium involving AGROGENE. These loci were amplified in 41 Spanish cultivars of V. vinifera. Eleven of the markers were polymorphic and informative in V. vinifera. Twelve were monomorphic and of the remaining markers one was polymorphic but less useful because individuals amplified more than two bands and the rest had amplification problems. The number of alleles detected for the 11 informative markers ranged from 4 to 12, with heterozygosity values ranging from 0.6 to 0.8. Primer sequences are reported for these markers.