Development and characterization of new microsatellite markers for grape

  1. Arroyo-García, R. 12
  2. Martínez-Zapater, J.M. 12
  1. 1 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  2. 2 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

Revista:
Vitis

ISSN: 0042-7500

Any de publicació: 2004

Volum: 43

Número: 4

Pàgines: 175-178

Tipus: Article

Altres publicacions en: Vitis

Repositori institucional: lock_openAccés obert Editor

Resum

Thirty five new grape microsatellite markers were developed under an international consortium involving AGROGENE. These loci were amplified in 41 Spanish cultivars of V. vinifera. Eleven of the markers were polymorphic and informative in V. vinifera. Twelve were monomorphic and of the remaining markers one was polymorphic but less useful because individuals amplified more than two bands and the rest had amplification problems. The number of alleles detected for the 11 informative markers ranged from 4 to 12, with heterozygosity values ranging from 0.6 to 0.8. Primer sequences are reported for these markers.