Characterization of genetic variation within table grape varieties (Vitis vinifera L.) based on AFLP markers

  1. Cervera, M.T. 1
  2. Cabezas, J.A. 1
  3. Sanchez-Escribano, E. 1
  4. Cenis, J.L. 1
  5. Martinez-Zapater, J.M. 1
  1. 1 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

Revista:
Vitis

ISSN: 0042-7500

Año de publicación: 2000

Volumen: 39

Número: 3

Páginas: 109-114

Tipo: Artículo

SCOPUS: 2-s2.0-0033776256 GOOGLE SCHOLAR

Otras publicaciones en: Vitis

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

AFLPs were used to characterize a collection of 35 table grape varieties. A single AFLP reaction with a single combination of selective primers yielded an average of 50 polymorphic and scorable amplified bands in this collection and was enough to unequivocally identify each analyzed variety. Genetic similarity between different varieties was estimated, based on the results obtained with two primer combinations, to be between 0.65 and 0.90, while sibling varieties derived from the same cross showed a genetic similarity higher than 0.80. The analysis of several samples belonging to the same varieties, yet from different origins, showed stability of the AFLP patterns as to unequivocally and reproducibly identify them. Furthermore, some specific primer combinations detected a low level of variation that could be due to somatic variation and would be useful in clonal identification.