Characterization of genetic variation within table grape varieties (Vitis vinifera L.) based on AFLP markers

  1. Cervera, M.T. 1
  2. Cabezas, J.A. 1
  3. Sanchez-Escribano, E. 1
  4. Cenis, J.L. 1
  5. Martinez-Zapater, J.M. 1
  1. 1 Centro Nacional de Biotecnología
    info

    Centro Nacional de Biotecnología

    Madrid, España

    ROR https://ror.org/015w4v032

Aldizkaria:
Vitis

ISSN: 0042-7500

Argitalpen urtea: 2000

Alea: 39

Zenbakia: 3

Orrialdeak: 109-114

Mota: Artikulua

SCOPUS: 2-s2.0-0033776256 GOOGLE SCHOLAR

Beste argitalpen batzuk: Vitis

Gordailu instituzionala: lock_openSarbide irekia Editor

Laburpena

AFLPs were used to characterize a collection of 35 table grape varieties. A single AFLP reaction with a single combination of selective primers yielded an average of 50 polymorphic and scorable amplified bands in this collection and was enough to unequivocally identify each analyzed variety. Genetic similarity between different varieties was estimated, based on the results obtained with two primer combinations, to be between 0.65 and 0.90, while sibling varieties derived from the same cross showed a genetic similarity higher than 0.80. The analysis of several samples belonging to the same varieties, yet from different origins, showed stability of the AFLP patterns as to unequivocally and reproducibly identify them. Furthermore, some specific primer combinations detected a low level of variation that could be due to somatic variation and would be useful in clonal identification.