Isolation and molecular characterization of the arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase

  1. Blázquez, M.A. 34
  2. Santos, E. 2
  3. Flores, C.-L. 13
  4. Martínez-Zapater, J.M. 2
  5. Salinas, J. 2
  6. Gancedo, C. 3
  1. 1 Universidad de La Habana
    info

    Universidad de La Habana

    La Habana, Cuba

    ROR https://ror.org/04204gr61

  2. 2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
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    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  3. 3 Instituto de Investigaciones Biomédicas Alberto Sols
    info

    Instituto de Investigaciones Biomédicas Alberto Sols

    Madrid, España

    ROR https://ror.org/00ha1f767

  4. 4 Plant Biology Laboratory, Salk Institute, 10010 N. Torrey Pines Rd., San Diego, CA 92037, United States
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Revista:
Plant Journal

ISSN: 0960-7412

Año de publicación: 1998

Volumen: 13

Número: 5

Páginas: 685-689

Tipo: Artículo

DOI: 10.1046/J.1365-313X.1998.00063.X PMID: 9681010 SCOPUS: 2-s2.0-0032033441 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Plant Journal

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

An Arabidopsis thailana cDNA clone, AtTPS1, that encodes a trehalose-6-phospheta synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate syntheses of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gens and is expressed constitutively at very low levels.