Isolation and molecular characterization of the arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase
- Blázquez, M.A. 34
- Santos, E. 2
- Flores, C.-L. 13
- Martínez-Zapater, J.M. 2
- Salinas, J. 2
- Gancedo, C. 3
-
1
Universidad de La Habana
info
-
2
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
info
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
Madrid, España
-
3
Instituto de Investigaciones Biomédicas Alberto Sols
info
- 4 Plant Biology Laboratory, Salk Institute, 10010 N. Torrey Pines Rd., San Diego, CA 92037, United States
ISSN: 0960-7412
Datum der Publikation: 1998
Ausgabe: 13
Nummer: 5
Seiten: 685-689
Art: Artikel
Andere Publikationen in: Plant Journal
Zusammenfassung
An Arabidopsis thailana cDNA clone, AtTPS1, that encodes a trehalose-6-phospheta synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate syntheses of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gens and is expressed constitutively at very low levels.