The distribution of 5-methylcytosine in the nuclear genome of plants

  1. Montero, L.M. 1
  2. Filipski, J. 12
  3. Gil, Pedro. 1
  4. Capel, J. 1
  5. Martinez-zapater, J.M. 1
  6. Salinas, J. 1
  1. 1 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  2. 2 Institut Jacques Monod
    info

    Institut Jacques Monod

    París, Francia

    ROR https://ror.org/02c5gc203

Revista:
Nucleic Acids Research

ISSN: 0305-1048

Año de publicación: 1992

Volumen: 20

Número: 12

Páginas: 3207-3210

Tipo: Artículo

PMID: 1620618 SCOPUS: 2-s2.0-0026684384 GOOGLE SCHOLAR

Otras publicaciones en: Nucleic Acids Research

Resumen

We have determined the 5-methylcytosine (5mC) content in high molecular weight DNA, from two dicot (tobacco and pea) and two monocot (wheat and maize) plant species, fractionated according to base composition. The results show that the proportion of 5mC in the genomic fractions increases linearly with their guanine + cytosine (G + C) content while the proportion of non-methylated cytosine remains almost constant. This can be interpreted as a consequence of a difference in mutation pressure related to spontaneous deamination of SmC to thymine between the different compartments of plant genomes.