The distribution of 5-methylcytosine in the nuclear genome of plants

  1. Montero, L.M. 1
  2. Filipski, J. 12
  3. Gil, Pedro. 1
  4. Capel, J. 1
  5. Martinez-zapater, J.M. 1
  6. Salinas, J. 1
  1. 1 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

  2. 2 Institut Jacques Monod
    info

    Institut Jacques Monod

    París, Francia

    ROR https://ror.org/02c5gc203

Zeitschrift:
Nucleic Acids Research

ISSN: 0305-1048

Datum der Publikation: 1992

Ausgabe: 20

Nummer: 12

Seiten: 3207-3210

Art: Artikel

PMID: 1620618 SCOPUS: 2-s2.0-0026684384 GOOGLE SCHOLAR

Andere Publikationen in: Nucleic Acids Research

Zusammenfassung

We have determined the 5-methylcytosine (5mC) content in high molecular weight DNA, from two dicot (tobacco and pea) and two monocot (wheat and maize) plant species, fractionated according to base composition. The results show that the proportion of 5mC in the genomic fractions increases linearly with their guanine + cytosine (G + C) content while the proportion of non-methylated cytosine remains almost constant. This can be interpreted as a consequence of a difference in mutation pressure related to spontaneous deamination of SmC to thymine between the different compartments of plant genomes.