Vancomycin-resistant enterococci from Portuguese wastewater treatment plant.

  1. Araújo, C. 2
  2. Torres, C. 1
  3. Silva, N. 2
  4. Carneiro, C. 2
  5. Gonçalves, A. 2
  6. Radhouani, H. 2
  7. Correia, S. 2
  8. Da Costa, P.M. 3
  9. Paccheco, R. 2
  10. Zarazaga, M. 1
  11. Ruiz-Larrea, F. 1
  12. Poeta, P. 2
  13. Igrejas, G. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidade Do Porto
    info

    Universidade Do Porto

    Oporto, Portugal

    ROR https://ror.org/043pwc612

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Revista:
Journal of Basic Microbiology

ISSN: 0233-111X

Año de publicación: 2010

Volumen: 50

Número: 6

Páginas: 605-609

Tipo: Artículo

DOI: 10.1002/JOBM.201000102 PMID: 20806259 SCOPUS: 2-s2.0-78650325769 WoS: WOS:000285427400012 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Basic Microbiology

Resumen

The objective of this study was to evaluate the incidence of vancomycin resistant enterococci in sludge and sewage of urban and poultry-slaughterhouse wastewater treatment plants. A total of 17 vancomycin resistant enterococci (eight vanA -containing Enterococcus faecium and nine vanC1/vanC2 -containing Enterococcus gallinarum/casseliflavus) were found among 499 isolates of sewage and sludge samples of 14 urban and nine poultry-slaughterhouse wastewater treatment plants. These seventeen VRE isolates showed resistance to kanamycin (n = 8), tetracycline (n = 7), erythromycin (n = 7), ciprofloxacin (n = 7), ampicillin (n = 7), streptomycin (n = 6), and gentamicin (n = 2). The tetM gene, related with tetracycline resistance, was found in six of eight van A-containing isolates, in all seven vanC-1 isolates and in one of two vanC-2 isolates. The ermB gene in seven erythromycin-resistant isolates; and the aac6 ′-aph2 ″ gene in the two high-level-gentamicin-resistant isolates. Moreover, two vanA -containing E. faecium isolates harbored the hyl virulence gene, and three isolates the entA bacteriocin gene. The purK-1 allele was detected in our urban vanA -containing E. faecium isolate, and we found as well the purK-6 allele in one poultry-slaughterhouse vanA -containing E. faecium isolate. This study suggests that the wastewater treatment plants might be an important source of dissemination of antibiotic-resistant enterococci in Portugal. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.