Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

  1. Koop, G. 6
  2. Vrieling, M. 19
  3. Storisteanu, D.M.L. 8
  4. Lok, L.S.C. 8
  5. Monie, T. 920
  6. Van Wigcheren, G. 19
  7. Raisen, C. 9
  8. Ba, X. 9
  9. Gleadall, N. 9
  10. Hadjirin, N. 9
  11. Timmerman, A.J. 6
  12. Wagenaar, J.A. 36
  13. Klunder, H.M. 6
  14. Fitzgerald, J.R. 22
  15. Zadoks, R. 1221
  16. Paterson, G.K. 22
  17. Torres, C. 2
  18. Waller, A.S. 1
  19. Loeffler, A. 5
  20. Loncaric, I. 13
  21. Hoet, A.E. 11
  22. Bergström, K. 4
  23. De Martino, L. 10
  24. Pomba, C. 16
  25. De Lencastre, H. 1718
  26. Ben Slama, K. 15
  27. Gharsa, H. 15
  28. Richardson, E.J. 14
  29. Chilvers, E.R. 8
  30. De Haas, C. 19
  31. Van Kessel, K. 19
  32. Van Strijp, J.A.G. 19
  33. Harrison, E.M. 7
  34. Holmes, M.A. 9
  35. Mostrar todos los/as autores/as +
  1. 1 Animal Health Trust, Lanwades Park, Kentford, Newmarket, United Kingdom
  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Central Veterinary Institute of Wageningen UR, Lelystad, Netherlands
  4. 4 Department of Animal Health and Antimicrobial Strategies, SVA, Uppsala, Sweden
  5. 5 Royal Veterinary College
    info

    Royal Veterinary College

    Londres, Reino Unido

    ROR https://ror.org/01wka8n18

  6. 6 Utrecht University
    info

    Utrecht University

    Utrecht, Holanda

    ROR https://ror.org/04pp8hn57

  7. 7 Addenbrooke's Hospital
    info

    Addenbrooke's Hospital

    Cambridge, Reino Unido

    ROR https://ror.org/055vbxf86

  8. 8 Papworth Hospital
    info

    Papworth Hospital

    Cambridge, Reino Unido

    ROR https://ror.org/05mqgrb58

  9. 9 University of Cambridge
    info

    University of Cambridge

    Cambridge, Reino Unido

    ROR https://ror.org/013meh722

  10. 10 University of Naples Federico II
    info

    University of Naples Federico II

    Nápoles, Italia

    ROR https://ror.org/05290cv24

  11. 11 Ohio State University
    info

    Ohio State University

    Columbus, Estados Unidos

    ROR https://ror.org/00rs6vg23

  12. 12 University of Glasgow
    info

    University of Glasgow

    Glasgow, Reino Unido

    ROR https://ror.org/00vtgdb53

  13. 13 University of Veterinary Medicine Vienna
    info

    University of Veterinary Medicine Vienna

    Viena, Austria

    ROR https://ror.org/01w6qp003

  14. 14 University of Birmingham
    info

    University of Birmingham

    Birmingham, Reino Unido

    ROR https://ror.org/03angcq70

  15. 15 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  16. 16 Universidade de Lisboa
    info

    Universidade de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/01c27hj86

  17. 17 Universidade Nova de Lisboa
    info

    Universidade Nova de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/02xankh89

  18. 18 Rockefeller University
    info

    Rockefeller University

    Nueva York, Estados Unidos

    ROR https://ror.org/0420db125

  19. 19 University Medical Center Utrecht
    info

    University Medical Center Utrecht

    Utrecht, Holanda

    ROR https://ror.org/0575yy874

  20. 20 MRC Human Nutrition Research
    info

    MRC Human Nutrition Research

    Cambridge, Reino Unido

    ROR https://ror.org/050pqs331

  21. 21 Moredun Research Institute, Bush Loan, Penicuik, United Kingdom
  22. 22 University of Edinburgh
    info

    University of Edinburgh

    Edimburgo, Reino Unido

    ROR https://ror.org/01nrxwf90

Mostrar afiliaciones +
Revista:
Scientific Reports

ISSN: 2045-2322

Año de publicación: 2017

Volumen: 7:40660

Páginas: 1-10

Tipo: Artículo

DOI: 10.1038/SREP40660 SCOPUS: 2-s2.0-85010041795 WoS: WOS:000392344800001 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Scientific Reports

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.