Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus
- Koop, G. 6
- Vrieling, M. 19
- Storisteanu, D.M.L. 8
- Lok, L.S.C. 8
- Monie, T. 920
- Van Wigcheren, G. 19
- Raisen, C. 9
- Ba, X. 9
- Gleadall, N. 9
- Hadjirin, N. 9
- Timmerman, A.J. 6
- Wagenaar, J.A. 36
- Klunder, H.M. 6
- Fitzgerald, J.R. 22
- Zadoks, R. 1221
- Paterson, G.K. 22
- Torres, C. 2
- Waller, A.S. 1
- Loeffler, A. 5
- Loncaric, I. 13
- Hoet, A.E. 11
- Bergström, K. 4
- De Martino, L. 10
- Pomba, C. 16
- De Lencastre, H. 1718
- Ben Slama, K. 15
- Gharsa, H. 15
- Richardson, E.J. 14
- Chilvers, E.R. 8
- De Haas, C. 19
- Van Kessel, K. 19
- Van Strijp, J.A.G. 19
- Harrison, E.M. 7
- Holmes, M.A. 9
- Mostrar todos os autores +
- 1 Animal Health Trust, Lanwades Park, Kentford, Newmarket, United Kingdom
-
2
Universidad de La Rioja
info
- 3 Central Veterinary Institute of Wageningen UR, Lelystad, Netherlands
- 4 Department of Animal Health and Antimicrobial Strategies, SVA, Uppsala, Sweden
-
5
Royal Veterinary College
info
-
6
Utrecht University
info
-
7
Addenbrooke's Hospital
info
-
8
Papworth Hospital
info
-
9
University of Cambridge
info
-
10
University of Naples Federico II
info
-
11
Ohio State University
info
-
12
University of Glasgow
info
-
13
University of Veterinary Medicine Vienna
info
-
14
University of Birmingham
info
-
15
Université de Tunis El Manar
info
-
16
Universidade de Lisboa
info
-
17
Universidade Nova de Lisboa
info
-
18
Rockefeller University
info
-
19
University Medical Center Utrecht
info
-
20
MRC Human Nutrition Research
info
- 21 Moredun Research Institute, Bush Loan, Penicuik, United Kingdom
-
22
University of Edinburgh
info
ISSN: 2045-2322
Ano de publicación: 2017
Volume: 7:40660
Páxinas: 1-10
Tipo: Artigo
Outras publicacións en: Scientific Reports
Resumo
Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.