Pseudomonas aeruginosa Isolates from Spanish Children: Occurrence in Faecal Samples, Antimicrobial Resistance, Virulence, and Molecular Typing

  1. Ruiz-Roldán, L. 2
  2. Bellés, A. 5
  3. Bueno, J. 5
  4. Azcona-Gutiérrez, J.M. 3
  5. Rojo-Bezares, B. 2
  6. Torres, C. 12
  7. Castillo, F.J. 45
  8. Sáenz, Y. 2
  9. Seral, C. 45
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  4. 4 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

  5. 5 Hospital Clínico Universitario Lozano Blesa
    info

    Hospital Clínico Universitario Lozano Blesa

    Zaragoza, España

    ROR https://ror.org/03fyv3102

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Revista:
BioMed Research International

ISSN: 2314-6133

Año de publicación: 2018

Volumen: 2018

Tipo: Artículo

DOI: 10.1155/2018/8060178 SCOPUS: 2-s2.0-85049343552 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: BioMed Research International

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Pseudomonas aeruginosa is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by P. aeruginosa isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of P. aeruginosa from faecal samples of children from two different Spanish regions were characterised. Seventy-two P. aeruginosa were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 exoU-positive strains. Alteration in protein OprD showed eight different patterns. The unique imipenem-resistant strain showed a premature stop codon in OprD. Intestinal colonisation by P. aeruginosa, mainly by international clones (as ST244, ST253, and ST274), is an important factor for the systemic infections development and the environmental dissemination. Periodic active surveillance is useful to identify these community human reservoirs and to control the evolution of antibiotic resistance and virulence activity. © 2018 Lidia Ruiz-Roldán et al.