Pseudomonas aeruginosa Isolates from Spanish Children: Occurrence in Faecal Samples, Antimicrobial Resistance, Virulence, and Molecular Typing
- Ruiz-Roldán, L. 2
- Bellés, A. 5
- Bueno, J. 5
- Azcona-Gutiérrez, J.M. 3
- Rojo-Bezares, B. 2
- Torres, C. 12
- Castillo, F.J. 45
- Sáenz, Y. 2
- Seral, C. 45
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1
Universidad de La Rioja
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2
Centro de Investigación Biomédica de La Rioja
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3
Hospital San Pedro
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4
Universidad de Zaragoza
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5
Hospital Clínico Universitario Lozano Blesa
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ISSN: 2314-6133
Argitalpen urtea: 2018
Alea: 2018
Mota: Artikulua
beta Ver similares en nube de resultadosBeste argitalpen batzuk: BioMed Research International
Laburpena
Pseudomonas aeruginosa is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by P. aeruginosa isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of P. aeruginosa from faecal samples of children from two different Spanish regions were characterised. Seventy-two P. aeruginosa were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 exoU-positive strains. Alteration in protein OprD showed eight different patterns. The unique imipenem-resistant strain showed a premature stop codon in OprD. Intestinal colonisation by P. aeruginosa, mainly by international clones (as ST244, ST253, and ST274), is an important factor for the systemic infections development and the environmental dissemination. Periodic active surveillance is useful to identify these community human reservoirs and to control the evolution of antibiotic resistance and virulence activity. © 2018 Lidia Ruiz-Roldán et al.