Culture media and screening methods for detection of vancomycin resistance in Enterococcus.

  1. Torres, C. 1
  2. Tenorio, C. 1
  3. Zarazaga, M. 1
  4. Baquero, F. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

Revista:
Revista Española de Quimioterapia

ISSN: 0214-3429

Año de publicación: 1996

Volumen: 9

Número: 1

Páginas: 45-48

Tipo: Artículo

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Otras publicaciones en: Revista Española de Quimioterapia

Resumen

Two hundred and twenty-nine enterococcal strains were studied to evaluate the influence of culture media and screening methods for detection of different levels of vancomycin resistance. Mueller-Hinton or Brain-Heart Infusion agar plates containing a concentration of 4 μg/ml of vancomycin with an inoculum of 104 cfu/spot reliably detected intermediate or resistant enterococcal isolates. Intermediate strain detection by disk diffusion tests in Mueller-Hinton agar was suboptimal; the use of Brain-Heart Infusion agar erroneously categorized as intermediate 40% of the susceptible strains.