Culture media and screening methods for detection of vancomycin resistance in Enterococcus.
- Torres, C. 1
- Tenorio, C. 1
- Zarazaga, M. 1
- Baquero, F. 1
-
1
Universidad de La Rioja
info
ISSN: 0214-3429
Any de publicació: 1996
Volum: 9
Número: 1
Pàgines: 45-48
Tipus: Article
Altres publicacions en: Revista Española de Quimioterapia
Resum
Two hundred and twenty-nine enterococcal strains were studied to evaluate the influence of culture media and screening methods for detection of different levels of vancomycin resistance. Mueller-Hinton or Brain-Heart Infusion agar plates containing a concentration of 4 μg/ml of vancomycin with an inoculum of 104 cfu/spot reliably detected intermediate or resistant enterococcal isolates. Intermediate strain detection by disk diffusion tests in Mueller-Hinton agar was suboptimal; the use of Brain-Heart Infusion agar erroneously categorized as intermediate 40% of the susceptible strains.