Diversity of structures carrying the aac(6`)-aph(2") gene in clinical Enterococcus faecalis and Enterococcus faecium strains isolated in Tunisia

  1. Klibi, N. 3
  2. Ben Slama, K. 3
  3. Masmoudi, A. 2
  4. Gharbi, S. 3
  5. Ruiz-Larrea, F. 1
  6. Fendri, C. 2
  7. Boudabous, A. 3
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hôpital La Rabta
    info

    Hôpital La Rabta

    Túnez, Túnez

    ROR https://ror.org/00gffbx54

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2006

Volumen: 18

Número: 4

Páginas: 353-359

Tipo: Artículo

PMID: 17024789 SCOPUS: 2-s2.0-33749017034 WoS: WOS:000240697300002 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

Resumen

The diversity of structures carrying the aac(6′)-aph(2″) gene was studied in 46 high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium clinical strains recovered in a Tunisian hospital during the period 2000-2003. The inclusion of the aac(6′)-aph(2″) gene within the Tn4001 composite element or in its truncated forms (lacking the IS256 at the right, the left or at both sides of the aac(6′)-aph(2″) gene) was investigated by PCR and sequencing. The aac(6′)-aph(2″) gene was included in the composite Tn4001 element in 19 of 34 high-level gentamicin-resistant E. faecalis strains (56%) and in 1 of 12 E. faecium strains (12%). A truncated form of Tn4001 lacking IS256 at the left-hand (in 10 E. faecalis and 8 E. faecium), at the right-hand (3 E. faecalis and 2 E. faecium) or at both sides of the aac(6′)-aph(2″) gene (in 2 E. faecalis and 1 E. faecium) was also detected in 26 of our enterococci. The transference by conjugation of the aac(6′)-aph(2″) gene, associated with other resistance genes, was demonstrated in seven of the high-level gentamicin-resistant E. faecalis strains. © E.S.I.F.T. srl.