Determination of trans-Resveratrol and Other Polyphenols in Wines by a Continuous Flow Sample Clean-up System followed by Capillary Electrophoresis Separation
- Arce, L. 1
- Teresa Tena, M. 1
- Rios, A. 1
- Valcárcel, M. 1
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1
Universidad de Córdoba
info
ISSN: 0003-2670
Argitalpen urtea: 1998
Alea: 359
Zenbakia: 1-2
Orrialdeak: 27-38
Mota: Artikulua
beta Ver similares en nube de resultadosBeste argitalpen batzuk: Analytica Chimica Acta
Laburpena
A new method coupling flow injection (FI) with capillary electrophoresis (CE) has been developed using diode array detection to measure the concentration of trans-resveratrol in wines, in particular because of the interest in its biological properties and cancer prevention. A FI system furnished with a C-18 minicolumn was used to clean up the wines by solid phase extraction prior to CE. The analytes were eluted from C-18 by using methanol and then driven from the FI system to the autosampler of the CE equipment by a programmable arm. The 3σ detection limit ranged from 0.05 mg l-1 (trans-resveratrol) to 0.36 mg l-1 [(-)epicatechin]. The recoveries of added trans-resveratrol and other polyphenols from synthetic wines were between 92% to 110%, (mean of 99%). The method is faster and simpler than those previously reported which used liquid-liquid extraction and liquid chromatography.