Determination of trans-Resveratrol and Other Polyphenols in Wines by a Continuous Flow Sample Clean-up System followed by Capillary Electrophoresis Separation

  1. Arce, L. 1
  2. Teresa Tena, M. 1
  3. Rios, A. 1
  4. Valcárcel, M. 1
  1. 1 Universidad de Córdoba
    info

    Universidad de Córdoba

    Córdoba, España

    ROR https://ror.org/05yc77b46

Revista:
Analytica Chimica Acta

ISSN: 0003-2670

Año de publicación: 1998

Volumen: 359

Número: 1-2

Páginas: 27-38

Tipo: Artículo

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DOI: 10.1016/S0003-2670(97)00668-5 SCOPUS: 2-s2.0-0032546079 GOOGLE SCHOLAR

Otras publicaciones en: Analytica Chimica Acta

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

A new method coupling flow injection (FI) with capillary electrophoresis (CE) has been developed using diode array detection to measure the concentration of trans-resveratrol in wines, in particular because of the interest in its biological properties and cancer prevention. A FI system furnished with a C-18 minicolumn was used to clean up the wines by solid phase extraction prior to CE. The analytes were eluted from C-18 by using methanol and then driven from the FI system to the autosampler of the CE equipment by a programmable arm. The 3σ detection limit ranged from 0.05 mg l-1 (trans-resveratrol) to 0.36 mg l-1 [(-)epicatechin]. The recoveries of added trans-resveratrol and other polyphenols from synthetic wines were between 92% to 110%, (mean of 99%). The method is faster and simpler than those previously reported which used liquid-liquid extraction and liquid chromatography.