Detection of clonally-related vanB2-containing Enterococcus faecium strains in two Spanish Hospitals

  1. Torres, C. 1
  2. Escobar, S. 4
  3. Portillo, A. 12
  4. Torres, L. 4
  5. Rezusta, A. 4
  6. Ruiz-Larrea, F. 1
  7. Revillo, M.J. 4
  8. Aspiroz, C. 3
  9. Zarazaga, M. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  3. 3 Hospital Royo Villanova, Zaragoza, Spain
  4. 4 Hospital Miguel Servet
    info

    Hospital Miguel Servet

    Zaragoza, España

    ROR https://ror.org/01r13mt55

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Revue:
Journal of Medical Microbiology

ISSN: 0022-2615

Année de publication: 2006

Volumen: 55

Número: 9

Pages: 1237-1243

Type: Article

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DOI: 10.1099/JMM.0.46560-0 PMID: 16914654 SCOPUS: 2-s2.0-33748568809 WoS: WOS:000240606900011 GOOGLE SCHOLAR

D'autres publications dans: Journal of Medical Microbiology

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Résumé

The aim of this study was to characterize the resistance mechanism in four clinical and five intestinal vancomycin-resistant Enterococcus faecium strains with VanB phenotype recovered from unrelated patients confined in two Spanish hospitals and to determine their clonal relationships. MIC values for vancomycin and teicoplanin were 16-32 and 0.5 μg ml -1, respectively. The mechanism of vancomycin resistance, as well as the genetic environment of the implicated gene, was analysed by PCR and sequencing. The vanB2 gene was detected in all nine E. faecium strains and the intergenic vanS B-Y B region showed the characteristic mutations of the vanB2 subtype. Two possibly related PFGE patterns, A (seven strains) and B (two strains), were distinguished among these enterococci. The vanX B-ORFC intergenic region was amplified in the nine strains and two amino acid changes were detected in the protein encoded by the vanX B gene in strains of pattern A with respect to those of pattern B. The vanB2 gene cluster was integrated into Tn5382 in all nine strains, being pbp5 gene-linked to this transposon. The ant(6′)-Ia, aph(3′)-IIIa and erm(B) genes were also detected in all of the strains. Both isolates with PFGE pattern B contained the esp gene. In summary, vanB2-containing E. faecium strains with indistinguishable PFGE patterns were recovered from seven patients from two Spanish hospitals. © 2006 SGM.