Polymorphisms of pbp5 gene and correlation with ampicillin resistance in Enterococcus faecium isolates of animal origin
- Poeta, P. 123
- Costa, D. 13
- Igrejas, G. 3
- Sáenz, Y. 1
- Zarazaga, M. 1
- Rodrigues, J. 23
- Torres, C. 1
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1
Universidad de La Rioja
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- 2 Centro de Estudos de Ciências Animais e Veterinárias, Vila Real, Portugal
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3
Universidade de Trás-os-Montes e Alto Douro
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ISSN: 0022-2615
Année de publication: 2007
Volumen: 56
Número: PART 2
Pages: 236-240
Type: Article
beta Ver similares en nube de resultadosD'autres publications dans: Journal of Medical Microbiology
Résumé
The C-terminal region of the pbp5 gene was sequenced in 11 ampicillin-resistant and 5 ampicillin-susceptible Enterococcus faecium isolates of animal origin, and compared with a pbp5 reference sequence (GenBank accession no. X84860). Eight different pbp5 alleles (designated A-H) were detected when amino acid changes in the region 461-629 were considered. Three of these alleles (A-C) were detected in ampicillin-susceptible isolates (MIC range 1-8 μg ml-1), and included the changes 470H→Q, 471V→I, 487Q→L, 581I→V, 595E→A or 622E→D. The remaining five alleles (D-H) were found in ampicillin-resistant isolates (MIC range 32-256 μg ml-1); three of these alleles (F-H) presented a serine insertion at position 466′, in addition to other important amino acid changes (485M→A, 496N→K, 499A→T, 525E→D, 586V→L or 629E→V). The other two alleles presented the amino acid changes 496N→K and 629E→V (allele D), and 470H→Q (allele F). A correlation between deduced amino acid changes in PBP5 and ampicillin MICs was detected in animal E. faecium isolates. © 2007 SGM.