Polymorphisms of pbp5 gene and correlation with ampicillin resistance in Enterococcus faecium isolates of animal origin

  1. Poeta, P. 123
  2. Costa, D. 13
  3. Igrejas, G. 3
  4. Sáenz, Y. 1
  5. Zarazaga, M. 1
  6. Rodrigues, J. 23
  7. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Estudos de Ciências Animais e Veterinárias, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Journal:
Journal of Medical Microbiology

ISSN: 0022-2615

Year of publication: 2007

Volume: 56

Issue: PART 2

Pages: 236-240

Type: Article

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DOI: 10.1099/JMM.0.46778-0 PMID: 17244806 SCOPUS: 2-s2.0-33847223862 WoS: WOS:000244402500014 GOOGLE SCHOLAR

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Abstract

The C-terminal region of the pbp5 gene was sequenced in 11 ampicillin-resistant and 5 ampicillin-susceptible Enterococcus faecium isolates of animal origin, and compared with a pbp5 reference sequence (GenBank accession no. X84860). Eight different pbp5 alleles (designated A-H) were detected when amino acid changes in the region 461-629 were considered. Three of these alleles (A-C) were detected in ampicillin-susceptible isolates (MIC range 1-8 μg ml-1), and included the changes 470H→Q, 471V→I, 487Q→L, 581I→V, 595E→A or 622E→D. The remaining five alleles (D-H) were found in ampicillin-resistant isolates (MIC range 32-256 μg ml-1); three of these alleles (F-H) presented a serine insertion at position 466′, in addition to other important amino acid changes (485M→A, 496N→K, 499A→T, 525E→D, 586V→L or 629E→V). The other two alleles presented the amino acid changes 496N→K and 629E→V (allele D), and 470H→Q (allele F). A correlation between deduced amino acid changes in PBP5 and ampicillin MICs was detected in animal E. faecium isolates. © 2007 SGM.