Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres

  1. Klibi, N. 3
  2. Ben Slimen, N. 3
  3. Fhoula, I. 3
  4. López, M. 1
  5. Ben Slama, K. 3
  6. Daffonchio, D. 2
  7. Boudabous, A. 3
  8. Torres, C. 1
  9. Ouzari, H. 3
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 University of Milan
    info

    University of Milan

    Milán, Italia

    ROR https://ror.org/00wjc7c48

  3. 3 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

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Revista:
Microbes and Environments

ISSN: 1342-6311

Año de publicación: 2012

Volumen: 27

Número: 4

Páginas: 533-537

Tipo: Artículo

DOI: 10.1264/JSME2.ME12041 SCOPUS: 2-s2.0-84871170107 WoS: WOS:000312162800029 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Microbes and Environments

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.