Characterization of a new organization of the plantaricin locus in the inducible bacteriocin-producing Lactobacillus plantarum J23 of grape must origin

  1. Rojo-Bezares, B. 1
  2. Sáenz, Y. 1
  3. Navarro, L. 1
  4. Jiménez-Díaz, R. 2
  5. Zarazaga, M. 1
  6. Ruiz-Larrea, F. 1
  7. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Instituto de la Grasa
    info

    Instituto de la Grasa

    Sevilla, España

    ROR https://ror.org/00fkwx227

Revista:
Archives of Microbiology

ISSN: 0302-8933

Año de publicación: 2008

Volumen: 189

Número: 5

Páginas: 491-499

Tipo: Artículo

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DOI: 10.1007/S00203-007-0342-6 PMID: 18193201 SCOPUS: 2-s2.0-42449132832 WoS: WOS:000255089700007 GOOGLE SCHOLAR

Otras publicaciones en: Archives of Microbiology

Resumen

Lactobacillus plantarum J23 was previously characterized as a bacteriocin-producer-strain when it was cocultured with other lactic acid bacteria. In this work, the genetic organization of the pln locus in the J23 strain was studied and compared with those of previously described L. plantarum C11, WCFS1 and NC8 strains. A new organization of the plantaricin locus was detected in the J23 strain. The sequenced fragment (20,266 bp) comprised plnJLR, plnMNOP, plnEFI, plnGHSTUVWXY, and plNC8IF-plNC8HK-plnD operons, as well as a new region that includes three new orfs (GenBank accession number DQ323671). When the J23 pln gene sequences were compared with those included in the GenBank database, the identity of the putative encoded proteins was in the range 67.1-100%. The regulatory system and the repertoire of putative bacteriocins of the J23 pln locus presented important differences with respect to the ones of C11, WCFS1 and NC8, such as the absence of plnK and the presence of a larger plnJ gene than the previously described for the other L. plantarum strains. The pln locus in L. plantarum strains seems to be a mosaic-like structure with different modules and reorganizations that presents highly conserved regions related to transport and bacteriocin maturation and variable regions related to regulation and bacteriocin production. © 2007 Springer-Verlag.