Oxetane Grafts Installed Site-Selectively on Native Disulfides to Enhance Protein Stability and Activity In Vivo
- Martínez-Sáez, N. 1
- Sun, S. 1
- Oldrini, D. 3
- Sormanni, P. 1
- Boutureira, O. 1
- Carboni, F. 3
- Compañón, I. 2
- Deery, M.J. 1
- Vendruscolo, M. 1
- Corzana, F. 2
- Adamo, R. 3
- Bernardes, G.J.L. 14
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1
University of Cambridge
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2
Universidad de La Rioja
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- 3 GSK Vaccines, Via Fiorentina 1, Siena, Italy
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4
Universidade de Lisboa
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ISSN: 1433-7851
Año de publicación: 2017
Volumen: 56
Número: 47
Páginas: 14963-14967
Tipo: Artículo
beta Ver similares en nube de resultadosOtras publicaciones en: Angewandte Chemie International
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Resumen
A four-membered oxygen ring (oxetane) can be readily grafted into native peptides and proteins through site-selective bis-alkylation of cysteine residues present as disulfides under mild and biocompatible conditions. The selective installation of the oxetane graft enhances stability and activity, as demonstrated for a range of biologically relevant cyclic peptides, including somatostatin, proteins, and antibodies, such as a Fab arm of the antibody Herceptin and a designed antibody DesAb-Aβ against the human Amyloid-β peptide. Oxetane grafting of the genetically detoxified diphtheria toxin CRM197 improves significantly the immunogenicity of this protein in mice, which illustrates the general utility of this strategy to modulate the stability and biological activity of therapeutic proteins containing disulfides in their structures. © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.