Ion-Pair-Supercritical Fluid Extraction of Clenbuterol from Food Samples
- Jimenez-Carmona, M.M. 1
- Tena, M.T. 1
- de Castro, M.D.L. 1
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1
Universidad de Córdoba
info
ISSN: 0021-9673
Año de publicación: 1995
Volumen: 711
Número: 2
Páginas: 269-276
Tipo: Artículo
Otras publicaciones en: Journal of Chromatography A
Resumen
Simultaneous ion-pair/supercritical fluid extraction (SFE) was investigated as an alternative to liquid solvent extraction for clenbuterol determination. Clenbuterol was extracted from diatomaceous earth and food matrices (feedstuff, lyophilized milk and liver) as its 10-camphorsulfonate ion-pair using supercritical (SC) CO 2 for 30 min of dynamic extraction at 383 bar and 40°C. The ion-pair forming reagent was added to the extraction cell prior to SFE. Recoveries ranged from 12 to 87% with mean R.S.D. of 15% for triplicate extractions. The use of the ion-pair reagent as a CO 2 modifier was also studied in depth.