Ion-Pair-Supercritical Fluid Extraction of Clenbuterol from Food Samples

  1. Jimenez-Carmona, M.M. 1
  2. Tena, M.T. 1
  3. de Castro, M.D.L. 1
  1. 1 Universidad de Córdoba
    info

    Universidad de Córdoba

    Córdoba, España

    ROR https://ror.org/05yc77b46

Journal:
Journal of Chromatography A

ISSN: 0021-9673

Year of publication: 1995

Volume: 711

Issue: 2

Pages: 269-276

Type: Article

DOI: 10.1016/0021-9673(95)00526-S SCOPUS: 2-s2.0-0029083296 GOOGLE SCHOLAR

More publications in: Journal of Chromatography A

Institutional repository: lock_openOpen access Editor

Abstract

Simultaneous ion-pair/supercritical fluid extraction (SFE) was investigated as an alternative to liquid solvent extraction for clenbuterol determination. Clenbuterol was extracted from diatomaceous earth and food matrices (feedstuff, lyophilized milk and liver) as its 10-camphorsulfonate ion-pair using supercritical (SC) CO 2 for 30 min of dynamic extraction at 383 bar and 40°C. The ion-pair forming reagent was added to the extraction cell prior to SFE. Recoveries ranged from 12 to 87% with mean R.S.D. of 15% for triplicate extractions. The use of the ion-pair reagent as a CO 2 modifier was also studied in depth.