Improved Supercritical Fluid Extraction of Sulphonamides
- Tena, M.T. 1
- Luque de Castro, M.D. 1
- Valcárcel, M. 1
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1
Universidad de Córdoba
info
ISSN: 0009-5893
Year of publication: 1995
Volume: 40
Issue: 3-4
Pages: 197-203
Type: Article
More publications in: Chromatographia
Abstract
Different ways used for enhancing the yield of sulphonamides leached from solid supports are reported. Supercritical CO 2 and methanol-modified CO 2 were used as extractants of the target analytes and the impregnation of the solid sample with buffer, derivatization of the analytes and ion-pair formation were assessed. Only the sulphonamide/tetramethylammonium ion-pairs are quantitatively extracted from the solid supports using pure supercritical CO 2, while the other modifications and the presence of a cosolvent lead to recoveries lower than 30% for most of the analytes. Individual separation/quantitation of the analytes was performed off-line using a liquid chromatograph.