Intestinal colonization by vanA or vanB2-containing enterococcal isolates of healthy animals in Spain
- Torres, C. 1
- Tenorio, C. 1
- Portillo, A. 1
- García, Maite A.. 1
- Martínez, Carolina. 1
- Del Campo, R. 1
- Ruiz-Larrea, F. 1
- Zarazaga, M. 1
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1
Universidad de La Rioja
info
ISSN: 1076-6294
Año de publicación: 2003
Volumen: 9 Suppl 1
Número: (Suppl 1:S)
Páginas: 47-52
Tipo: Artículo
beta Ver similares en nube de resultadosOtras publicaciones en: Microbial Drug Resistance
Resumen
Fecal samples of healthy animals (66 pigs, 22 pets) recovered during 1998 in La Rioja, Spain, were analyzed for vancomycin-resistant enterococci colonization. Vancomycin resistance mechanisms were analyzed by PCR and sequencing. vanA-containing enterococci were detected in 3 of 66 samples (4.5%) and 5 of 22 samples (22.7%) of the pig and pet samples, respectively. Seven unrelated pulsed-field gel electrophoresis (PFGE) patterns were detected among the 8 vanA isolates (7 Enterococcus faecium, 1 E. faecalis). The tet(M) gene was present in all eight vanA enterococcal isolates, while the erm(B) and aac(6')-Ie-aph(2")-Ia genes were detected in 6 and 3 isolates, respectively. Colonization by vanC-1-containing enterococci (E. gallinarum) was demonstrated in 3% and 4.5% of the pig and pet samples. The aac(6')-Ie-aph(2")-Ia, ant(6)-Ia, aph(3')-IIIa, erm(B) and tet(M) genes were identified in one of the E. gallinarum isolates from a pig fecal sample. One vanB2-containing E. hirae strain was detected in the fecal sample of a healthy pig. In this isolate, the vanB2 gene cluster was integrated into the Tn5382-like element, as demonstrated by specific PCRs and sequencing. The tet(M) and erm(B) genes were also detected in this isolate. This is the first report in which a vanB2-containing enterococci is detected in animals and in E. hirae.