Reversible photocontrol of peptide conformation with a rhodopsin-like photoswitch
- Blanco-Lomas, M. 1
- Samanta, S. 2
- Campos, P.J. 1
- Woolley, G.A. 2
- Sampedro, D. 1
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1
Universidad de La Rioja
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2
University of Toronto
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ISSN: 0002-7863
Ano de publicación: 2012
Volume: 134
Número: 16
Páxinas: 6960-6963
Tipo: Artigo
beta Ver similares en nube de resultadosOutras publicacións en: Journal of the American Chemical Society
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Resumo
Reversible photocontrol of biomolecules requires chromophores that can efficiently undergo large conformational changes upon exposure to wavelengths of light that are compatible with living systems. We designed a benzylidene-pyrroline chromophore that mimics the Schiff base of rhodopsin and can be used to introduce light-switchable intramolecular cross-links in peptides and proteins. This new class of photoswitch undergoes an ∼10 Å change in end-to-end distance upon isomerization and can be used to control the conformation of a target peptide efficiently and reversibly using, alternately, violet (400 nm) and blue (446 nm) light. © 2012 American Chemical Society.