Surface glycosaminoglycans protect eukaryotic cells against membrane-driven peptide bacteriocins

  1. Martín, R. 1
  2. Escobedo, S 1
  3. Martín, Carla 1
  4. Crespo, A 13
  5. Quiros, L.M 1
  6. Suarez, J.E 12
  1. 1 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

  2. 2 Instituto de Productos Lácteos de Asturias
    info

    Instituto de Productos Lácteos de Asturias

    Villaviciosa, España

    ROR https://ror.org/00bnagp43

  3. 3 Neiker-Tecnalia Arkaute, Vitoria-Gasteiz, Spain
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Revista:
Antimicrobial Agents and Chemotherapy

ISSN: 0066-4804

Año de publicación: 2015

Volumen: 59

Número: 1

Páginas: 677

Tipo: Artículo

DOI: 10.1128/AAC.04427-14 PMID: 25331698 SCOPUS: 2-s2.0-84920172662 GOOGLE SCHOLAR

Otras publicaciones en: Antimicrobial Agents and Chemotherapy

Resumen

Enzymatic elimination of surface glycosaminoglycans or inhibition of their sulfation provokes sensitizing of HT-29 and HeLa cells toward the peptide bacteriocins nisin A, plantaricin C, and pediocin PA-1/AcH. The effect can be partially reversed by heparin, which also lowers the susceptibility of Lactococcus lactis to nisin A. These data indicate that the negative charge of the glycosaminoglycan sulfate residues binds the positively charged bacteriocins, thus protecting eukaryotic cells from plasma membrane damage. Copyright © 2015, American Society for Microbiology. All Rights Reserved.