Surface glycosaminoglycans protect eukaryotic cells against membrane-driven peptide bacteriocins
- Martín, R. 1
- Escobedo, S 1
- Martín, Carla 1
- Crespo, A 13
- Quiros, L.M 1
- Suarez, J.E 12
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1
Universidad de Oviedo
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2
Instituto de Productos Lácteos de Asturias
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- 3 Neiker-Tecnalia Arkaute, Vitoria-Gasteiz, Spain
ISSN: 0066-4804
Année de publication: 2015
Volumen: 59
Número: 1
Pages: 677
Type: Article
D'autres publications dans: Antimicrobial Agents and Chemotherapy
Résumé
Enzymatic elimination of surface glycosaminoglycans or inhibition of their sulfation provokes sensitizing of HT-29 and HeLa cells toward the peptide bacteriocins nisin A, plantaricin C, and pediocin PA-1/AcH. The effect can be partially reversed by heparin, which also lowers the susceptibility of Lactococcus lactis to nisin A. These data indicate that the negative charge of the glycosaminoglycan sulfate residues binds the positively charged bacteriocins, thus protecting eukaryotic cells from plasma membrane damage. Copyright © 2015, American Society for Microbiology. All Rights Reserved.