Bioaerosols chemometric characterization by laser-induced fluorescence: Air sample analysis
- Cabredo, S. 1
- Parra, A. 1
- Sáenz, C. 1
- Anzano, J. 2
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1
Universidad de La Rioja
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2
Universidad de Zaragoza
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ISSN: 0039-9140
Año de publicación: 2009
Volumen: 77
Número: 5
Páginas: 1837-1842
Tipo: Artículo
beta Ver similares en nube de resultadosOtras publicaciones en: Talanta (Oxford)
Resumen
A laser-induced fluorescence (LIF) system was optimized using a solution of Micrococcus luteus in ethanol/water 50% (v/v) to obtain spectra in the gas phase of 46 bioaerosols. Experimental designs such as Plackett-Burman and factorial design were applied. The fluorescence spectra were treated chemometrically by principal component analysis, linear discriminant analysis and hierarchical cluster analysis to classify the microorganisms according to family, morphology and gram. The best results were obtained using LDA. The method was applied to air samples and the LIF results allowed to characterize bioaerosols reliability. The robustness of the technique was demonstrated by the identification of many bacteria. © 2008 Elsevier B.V. All rights reserved.