Bioaerosols chemometric characterization by laser-induced fluorescence: Air sample analysis

  1. Cabredo, S. 1
  2. Parra, A. 1
  3. Sáenz, C. 1
  4. Anzano, J. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

Revista:
Talanta (Oxford)

ISSN: 0039-9140

Any de publicació: 2009

Volum: 77

Número: 5

Pàgines: 1837-1842

Tipus: Article

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DOI: 10.1016/J.TALANTA.2008.10.030 PMID: 19159807 SCOPUS: 2-s2.0-58149512093 WoS: WOS:000263388600044 GOOGLE SCHOLAR

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Resum

A laser-induced fluorescence (LIF) system was optimized using a solution of Micrococcus luteus in ethanol/water 50% (v/v) to obtain spectra in the gas phase of 46 bioaerosols. Experimental designs such as Plackett-Burman and factorial design were applied. The fluorescence spectra were treated chemometrically by principal component analysis, linear discriminant analysis and hierarchical cluster analysis to classify the microorganisms according to family, morphology and gram. The best results were obtained using LDA. The method was applied to air samples and the LIF results allowed to characterize bioaerosols reliability. The robustness of the technique was demonstrated by the identification of many bacteria. © 2008 Elsevier B.V. All rights reserved.