Proton nuclear magnetic resonance spectroscopy as a technique for gentamicin drug susceptibility studies with Escherichia coli ATCC 25922

  1. García-Álvarez, L. 1
  2. Busto, J.H. 2
  3. Avenoza, A. 2
  4. Sáenz, Y. 3
  5. Peregrina, J.M. 2
  6. Oteo, J.A. 1
  1. 1 Hospital San Pedro
    info

    Hospital San Pedro

    Logroño, España

    ROR https://ror.org/031va0421

  2. 2 Universidad de La Rioja
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    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

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Revista:
Journal of Clinical Microbiology

ISSN: 0095-1137

Año de publicación: 2015

Volumen: 53

Número: 8

Páginas: 2433-2438

Tipo: Artículo

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DOI: 10.1128/JCM.00604-15 SCOPUS: 2-s2.0-84938153078 WoS: WOS:000358290200008 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Clinical Microbiology

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Resumen

Antimicrobial drug susceptibility tests involving multiple time-consuming steps are still used as reference methods. Today, there is a need for the development of new automated instruments that can provide faster results and reduce operating time, reagent costs, and labor requirements. Nuclear magnetic resonance (NMR) spectroscopy meets those requirements. The metabolism and antimicrobial susceptibility of Escherichia coli ATCC 25922 in the presence of gentamicin have been analyzed using NMR and compared with a reference method. Direct incubation of the bacteria (with and without gentamicin) into the NMR tube has also been performed, and differences in the NMR spectra were obtained. The MIC, determined by the reference method found in this study, would correspond with the termination of the bacterial metabolism observed with NMR. Experiments carried out directly into the NMR tube enabled the development of antimicrobial drug susceptibility tests to assess the effectiveness of the antibiotic. NMR is an objective and reproducible method for showing the effects of a drug on the subject bacterium and can emerge as an excellent tool for studying bacterial activity in the presence of different antibiotic concentrations. Copyright © 2015, American Society for Microbiology. All Rights Reserved.