Evaluación de la eficacia de tres vacunas frente a Lactococcus garvieae en trucha arcoíris (Oncorhynchus mykiss) mediante reto por cohabitación.

  1. Isabel Fernandez Poch 3
  2. Tania Pérez-Sánchez 3
  3. Hector Fuertes 3
  4. Daniel Vendrell 1
  5. Imanol Ruiz-Zarzuela 3
  6. Francesc Padrós 2
  1. 1 Laboratorios Hipra, S.A., Amer, España.
  2. 2 Universitat Autònoma de Barcelona
    info

    Universitat Autònoma de Barcelona

    Barcelona, España

    ROR https://ror.org/052g8jq94

  3. 3 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

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Journal:
AquaTIC: revista electrónica de acuicultura

ISSN: 1578-4541

Year of publication: 2017

Issue: 47

Pages: 10-19

Type: Article

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More publications in: AquaTIC: revista electrónica de acuicultura

Abstract

Lactococcus garvieae (L. garvieae) is considered an emergent pathogen because of its zoonotic potential, an emergent pathogen (Meyburgh y cols., 2017) both in marine and freshwater aquaculture, and it causes important economic losses in summer, when water temperature rises (Vendrell y cols., 2006). Because of this, an adequate vaccination is the key to minimize the impact of that bacterium in those cultivated species that are more susceptible to his effects. In the present study, the efficacy of three L. garvieae vaccines was evaluated with a cohabitation infection 28 days post-vaccination. A commercial vaccine (Icthiovac LG) showed the best results with a RPS=87.50%, followed by an experimental trivalent vaccine (L. garvieae and two Y. ruckeri strains) with a RPS=81.25% and finally an experimental tetravalent vaccine (L. garvieae, two Y. ruckeri strains and Aeromonas salmonicida subsp. salmonicida) with a RPS=70.93%. The mortality results of each group was compared with those to the control group, indicating an increase of the immunity to this pathogen in all vaccinated groups (p<0.05).

Bibliographic References

  • Araújo C, Muñoz-Atienza E, Pérez-Sánchez T, Poeta P, Igrejas G, Hernández PE, Herranz C, Ruiz-Zarzuela I, Cintas LM (2015). Nisin Z Production by Lactococcus lactis subsp. cremoris WA2-67 of Aquatic Origin as a Defense Mechanism to Protect Rainbow Trout (Oncorhynchus mykiss, Walbaum) Against Lactococcus garvieae. Mar. Biotechnol. 17: 820–830.
  • Astorga N, Afonso JM, Zamorano MJ, Montero D, Oliva V, Fernández H, Izquierdo MS (2005). Evaluation of visible implant elastomer tags for tagging juvenile gilthead seabream (Sparus auratus L.); effects on growth, mortality, handling time and tag loss. Aquac. Res. 36: 733-738.
  • Bastardo A, Ravelo C, Castro N, Calheiros J, Romalde JL (2012). Effectiveness of bivalent vaccines against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynchus mykiss (Walbaum). Fish Shellfish Immunol. 32: 756-761.
  • Bercovier H., Ghittino C., Eldar A (1997). Immunization with bacterial antigens: infections with streptococci and related organisms. Dev. Biol. Stand. 90: 153-160.
  • Bruyndoncx l, Knaepkens G, Meeus W, Bervoets l, Eens M (2002). The evaluation of passive integrated transponder PIT tags and visible implant elastomer VIE marks as new marking techniques for the bullhead. J. Fish Biol. 60: 260-262.
  • Chettri JK, Skov J, Jaafar RM, Krossøy B, Kania PW, Dalsgaard I, Buchmann K (2015). Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout. Fish Shellfish Immunol. 44: 485-495.
  • Dewey MR, Zigler SJ (1996). An evaluation of Fluorescent Elastomer for Marking Bluegills in Experimental Studies. Prog. Fish Cult. 58: 219-220.
  • Featherstone ZL, Turnbull JF, Auchinachie NA, Crumlish M (2016). Evaluation of visible implant elastomer tags for pathogenesis research in Nile tilapia (Oreochromis niloticus). Aquac. Res. 47: 2419-2425.
  • Griffiths SP (2002). Retention of visible implant tags in small rockpool fishes. Mar. Ecol. Prog. Ser. 236: 307-309.
  • Hoel K, Salonius K, Lillehaug A (1997). Vibrio antigens of polyvalent vaccines enhance the humoral immune response to Aeromonas salmonicida antigens in Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol. 7: 71-80.
  • Hohn C, Petrie-Hanson L (2013). Evaluation of visible implant elastomer tags in zebrafish (Danio rerio). Open Biol. 000:1-5.
  • Jung MY, Chang YH, Kim W (2010). A real-time PCR assay for detection and quantification of Lactococcus garvieae. J. App. Microbiol. 108: 1694-1701.
  • Kubilay A, Altun S, Ulukoy G, Ekici S, Diler O (2008). Immunization of Rainbow trout (Oncorhynchus mykiss) against Lactococcus garvieae using vaccine mixures. Is. J. Aquacult-Bamid. 60(4): 268-273.
  • Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Meth. 25: 402-408.
  • Múzquiz JL, Royo FM, Ortega C, De Blas I, Ruiz I, Alonso JL (1999). Pathogenicity of streptococcosis in rainbow trout (Oncorhynchus mykiss): dependence of age of diseased fish. B. Eur. Assoc. Fish Pat. 19: 114–119.
  • Nikoskelainen S, Verho S, Järvinen S, Madetoja J, Wiklund T, Lilius E (2007). Multiple whole bacterial antigens in polyvalent vaccine may result in inhibition of specific responses in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 3: 206-217.
  • Nordmo R, Ramstad A (1997). Comparison of different challenge methods to evaluate the efficacy of furunculosis vaccines in Atlantic salmon, Salmo salar L. J. Fish Dis. 20: 119–126.
  • Pérez-Sánchez T, Balcázar JL, Merrifield D, Carnevali O, Gioacchini G, De Blas I, Ruiz-Zarzuela I (2011). Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection. Fish Shellfish Immunol. 31: 196-201.
  • Royo FM (1999). Estudio de la estreptococosis de la trucha arco iris (Oncorhynchus mykiss); factores implicados en su virulencia y patogenicidad. Aplicaciones para su control. Tesis, Universidad de Zaragoza, España.
  • Vendrell D, Balcázar JL, Ruiz-Zarzuela I, De Blas I, Gironés O, Múzquiz JL (2006). Lactococcus garvieae in fish: A review. Comp. Immunol. Microbiol. Infect. Dis. 29: 177–198.
  • Vendrell D, Balcázar JL, Ruiz-Zarzuela I, De Blas I, Gironés O, Múzquiz JL (2008). Safety and efficacy of an inactivated vaccine against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss). Prev. Vet. Med. 80: 222-229.
  • Zlotkin A, Eldar A, Ghittino C, Bercover H (1998). Identification of Lactococcus garvieae by PCR. J. Clin. Microbiol. 36: 983-985.
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