Ultrasound-assisted emulsification-microextraction for the sensitive determination of Brett character responsible compounds in wines.

  1. Pizarro, C. 1
  2. Sáenz-González, C. 1
  3. Pérez-del-Notario, N. 1
  4. González-Sáiz, J.M. 1
  1. 1 Universidad de La Rioja

    Universidad de La Rioja

    Logroño, España

    GRID grid.119021.a

Journal of Chromatography A

ISSN: 0021-9673

Year of publication: 2011

Volume: 1218

Issue: 50

Pages: 8975-8981

Type: Article

Export: RIS
DOI: 10.1016/j.chroma.2011.10.012 PMID: 22047819 SCOPUS: 2-s2.0-81855165041 WoS: 000298126900001 GOOGLE SCHOLAR


Cited by

  • Scopus Cited by: 22 (12-11-2021)

JCR (Journal Impact Factor)

  • Year 2011
  • Journal Impact Factor: 4.531
  • Best Quartile: Q1
  • Area: BIOCHEMICAL RESEARCH METHODS Quartile: Q1 Rank in area: 13/72 (Ranking edition: SCIE)
  • Area: CHEMISTRY, ANALYTICAL Quartile: Q1 Rank in area: 6/73 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2011
  • SJR Journal Impact: 2.451
  • Best Quartile: Q1
  • Area: Analytical Chemistry Quartile: Q1 Rank in area: 7/104
  • Area: Biochemistry Quartile: Q1 Rank in area: 42/402
  • Area: Organic Chemistry Quartile: Q1 Rank in area: 10/182
  • Area: Medicine (miscellaneous) Quartile: Q1 Rank in area: 100/2921


  • Year 2011
  • CiteScore of the Journal : 7.3
  • Area: Organic Chemistry Percentile: 91
  • Area: Analytical Chemistry Percentile: 90
  • Area: Medicine (all) Percentile: 88
  • Area: Biochemistry Percentile: 86


In this study, a method based on ultrasound-assisted emulsification-microextraction (USAEME) was optimised for the determination of Brett character responsible compounds (4-ethylguaiacol (EG), 4-ethylphenol (EP), 4-vinylguaiacol (VG) and 4-vinylphenol (VP)) in wines. For this purpose, once the extraction solvent had been selected, the influence on the extraction efficiency of ratio between extraction solvent and sample volumes, temperature, ionic strength and time was studied using experimental design methodology. The optimum conditions found for USAEME of 5. mL of wine were 160μL of chlorobenzene at 60°C during 5. min without salt addition. Then, the analytical performance of the optimised USAEME procedure was evaluated. For all the studied compounds, the proposed method showed correlation coefficients over 0.984. Satisfactory repeatability (below 10%) and inter-day repeatability (below 11%) were obtained at different concentration levels and the method achieved detection limits similar or even lower than previously reported for these compounds in wines. Moreover, the applicability of the method was evaluated by the analysis of real samples. To our knowledge, this is the first time that USAEME has been applied to the determination of Brett character responsible compounds in wine. © 2011 Elsevier B.V.