Resistance mechanisms and holding-level distribution of faecal Escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in Spain

  1. Escudero, E. 22
  2. Vinué, L. 12
  3. Teshager, T. 22
  4. Torres, C. 12
  5. Moreno, M.A. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Revista:
Research in Veterinary Science

ISSN: 0034-5288

Año de publicación: 2010

Volumen: 88

Número: 1

Páginas: 83-87

Tipo: Artículo

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DOI: 10.1016/J.RVSC.2009.05.021 PMID: 19577265 SCOPUS: 2-s2.0-71649091000 WoS: WOS:000273916000016 GOOGLE SCHOLAR

Otras publicaciones en: Research in Veterinary Science

Resumen

Introduction: Fecal Escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in Spain. The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level. Materials and methods: Twenty-nine E. coli isolates showing reduced susceptibility or resistance to extended-spectrum cephalosporins were collected from a sampling frame of 80 pig farms distributed over 13 Spanish provinces. The survey was carried out at the slaughterhouse level in 2004. Results: Of the 29 isolates, 21 (72%) met the criteria for a positive phenotypic confirmatory test for extended-spectrum beta-lactamases (ESBL). The following ESBLs were detected: SHV-12 (12 isolates, 41%), CTX-M-1 (three isolates, 10%), CTX-M-9 (three isolates, 10%), and CTX-M-14 (three isolates, 10%). The remaining eight isolates (28%) were phenotypically non-ESBL, with seven of them (24%) showing mutations on the chromosomal ampC gene promoter at positions -42 (C → T), -18 (G → A), -1 (C → T), and +58 (C → T). A multiplex PCR for detection of plasmidic class C beta-lactamases was negative for all isolates. Conclusion: Different ESBLs and other mechanisms linked to extended-spectrum cephalosporin resistance are widely distributed among fecal E. coli from slaughter pigs in Spain. © 2009 Elsevier Ltd. All rights reserved.