Genetic detection of extended-spectrum beta-lactamase-containing Escherichia coli isolates and vancomycin resistant enterococci in faecal samples of healthy children

  1. Guimarães, B. 23
  2. Barreto, Â. 23
  3. Radhouani, H. 23
  4. Figueiredo, N. 23
  5. Gaspar, E. 3
  6. Rodrigues, J. 23
  7. Torres, C. 1
  8. Igrejas, G. 3
  9. Poeta, P. 23
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2009

Volumen: 15

Número: 3

Páginas: 211-216

Tipo: Artículo

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DOI: 10.1089/MDR.2009.0910 SCOPUS: 2-s2.0-69949186446 WoS: WOS:000269536000010 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

Resumen

One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum β-lactamases (ESBLs) containing Escherichia coli isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive E. coli isolates and the bla CTX-M-1, bla TEM-52, and bla SHV-12 genes were identified in these isolates. The bla TEM-52-containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim-sulfamethoxazole, and chloramphenicol; sul1, sul3, and cmlA genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu+ Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate). vanA-containing Escherichia faecium isolates were detected in 13 of the 112 fecal samples (11.6%), and vanC-1 isolates were found in 2 samples. A diversity of resistance genes [(tet(M), tet(L), erm(B), aph(3′)-IIIa, ant(6)-Ia, catA, and vat(E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing E. coli and VRE isolates. © Copyright 2009, Mary Ann Liebert, Inc.