Prevalence and diversity of integrons and associated resistance genes in Escherichia coli isolates from poultry meat in Tunisia

  1. Soufi, L. 13
  2. Abbassi, M.S. 3
  3. Sáenz, Y. 12
  4. Vinué, L. 1
  5. Somalo, S. 1
  6. Zarazaga, M. 12
  7. Abbas, Asad. 1
  8. Dbaya, R. 3
  9. Khanfir, L. 3
  10. Ben Hassen, A. 4
  11. Hammami, S. 3
  12. Torres, C. 12
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Institute of Veterinary Research of Tunisia, Tunis, Tunisia
  4. 4 Laboratory of National Bone Marrow Transplantation Centre, Tunis, Tunisia
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Zeitschrift:
Foodborne Pathogens and Disease

ISSN: 1535-3141

Datum der Publikation: 2009

Ausgabe: 6

Nummer: 9

Seiten: 1067-1073

Art: Artikel

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DOI: 10.1089/FPD.2009.0284 PMID: 19642918 SCOPUS: 2-s2.0-70449350797 WoS: WOS:000271446900004 GOOGLE SCHOLAR

Andere Publikationen in: Foodborne Pathogens and Disease

Projekte im Zusammenhang

Evolución de la frecuencia y diversidad de integrones en escherichia coli de diferentes ecosistemas y su relación con la resistencia a los antimicrobianos

2006/00094/001

Zusammenfassung

Fifty-five Escherichia coli isolates were acquired from chicken and turkey meat obtained from two slaughterhouses in Tunis. Eighty-nine percent, 80%, 78%, 67%, 45%, 27%, 7%, 4%, and 2% of these isolates showed resistance to tetracycline, trimethoprim/sulfamethoxazole, streptomycin, nalidixic acid, ampicillin, chloramphenicol, ciprofloxacin, colistine, and gentamicin, respectively. No resistance was detected to cefotaxime, ceftazidime, or amikacin. bla TEM gene was found in 22 of 25 ampicillin-resistant isolates, and 1 isolate harbored bla OXA-1 gene. Tetracycline resistance was predominately mediated by the tetA gene. The sul1, sul2, and sul3 genes, alone or combined, were detected in 46 of 48 sulfonamide-resistant isolates, and sul1 and sul3 were included in class 1 integrons in some cases. Sixty percent of isolates harbored integrons (class 1, 30 isolates; class 2, 5 isolates). Class 2 integrons contained in all cases the dfrA1-sat1-aadA1-orfX gene cassette arrangement. Nine gene cassette arrangements have been detected among class 1 integrons, containing different alleles of dfrA (five alleles) and aadA (2 alleles) genes, which encode trimethoprim and streptomycin resistance, respectively. An uncommon gene cassette array (sat-psp-aadA2-cmlA1-aadA1-qacH- IS440-sul3) has been identified in three class 1 integron-positive isolates, and one additional isolate had this same structure with the insertion of IS26 inside the aadA1 gene (included in GenBank with accession no. FJ160769). The 55 studied isolates belong to the four phylogenic groups of E. coli, and phylogroups A and D were the most prevalent ones. At least one virulence-associated gene (fimA, papC, or aer) was detected in 44 of the 55 (80%) studied isolates. E. coli isolates of poultry origin could be a reservoir of antimicrobial-resistance genes and of integrons, and its evolution should be tracked in the future. © Copyright 2009, Mary Ann Liebert, Inc.