Wild boars as reservoirs of extended-spectrum beta-lactamases in Escherichia coli isolates of the A, B1 and B2 phylogenetic groups

  1. Poeta, P. 23
  2. Radhouani, H. 2
  3. Pinto, L. 2
  4. Martinho, A. 4
  5. Rego, V. 4
  6. Rodrigues, R. 4
  7. Gonçalves, A. 2
  8. Rodrigues, J. 23
  9. Estepa, V. 1
  10. Torres, C. 1
  11. Igrejas, G. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Center of Studies of Animal and Veterinary Sciences (CECAV), Vila Real, Portugal
  4. 4 Portugal North Forestry Resources, Vila Real, Portugal
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Revista:
Journal of Basic Microbiology

ISSN: 0233-111X

Año de publicación: 2009

Volumen: 49

Número: 6

Páginas: 584-588

Tipo: Artículo

DOI: 10.1002/JOBM.200900066 PMID: 19810044 SCOPUS: 2-s2.0-70749133617 WoS: WOS:000273186600011 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Basic Microbiology

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

ESBL-producing E. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in Portugal. The ESBL types identified by PCR and sequencing were blaCTX-M-1 (6 isolates) and blaCTX-M-1 + blaTEM1-b (2 isolates). Further resistance genes detected included tet(A) or tet(B) (in three tetracycline-resistant isolates), aadA (in three streptomycin- resistant isolates), cmlA (in one chloramphenicol-resistant isolate), sul1 and/or sul2 and/or sul3 (in all sulfonamide-resistant isolates). The intI1 gene encoding class 1 integrase was detected in all ESBL-producing E. coli isolates. One isolate also carried the intI2 gene, encoding class 2 integrase. The ESBL-producing E. coli isolates could be assigned to phylogenetic groups B1 (3 isolates), B2 (3 isolates) or A (2 isolates). Amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in three nalidixic acid-resistant and ciprofloxacin-susceptible isolates. Two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80Ile) were identified in two nalidixic acid- and ciprofloxacin-resistant isolates. As evidenced by this study wild boars could be a reservoir of antimicrobial resistance genes. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.