Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal

  1. Sousa, M. 1234
  2. Silva, N. 1
  3. Igrejas, G. 3
  4. Silva, F. 1
  5. Sargo, R. 1
  6. Alegria, N. 1
  7. Benito, D. 2
  8. Gómez, P. 2
  9. Lozano, C. 2
  10. Gómez-Sanz, E. 2
  11. Torres, C. 2
  12. Caniça, M. 4
  13. Poeta, P. 1
  1. 1 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Instituto de Biotecnologia e Bioengenharia
    info

    Instituto de Biotecnologia e Bioengenharia

    Lisboa, Portugal

    ROR https://ror.org/05ws8g470

  4. 4 National Institute of Health Dr. Ricardo Jorge (INSA), Lisboa, Portugal
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Revista:
Veterinary Microbiology

ISSN: 0378-1135

Año de publicación: 2014

Volumen: 171

Número: 3-4

Páginas: 436-440

Tipo: Artículo

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DOI: 10.1016/J.VETMIC.2014.02.034 SCOPUS: 2-s2.0-84901922691 WoS: WOS:000338391700024 GOOGLE SCHOLAR

Otras publicaciones en: Veterinary Microbiology

Repositorio institucional: lockAcceso abierto Editor

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2013/00001/001

Resumen

Antibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems. © 2014 Elsevier B.V. All rights reserved.