Comparative proteomics of an extended spectrum β-lactamase producing Escherichia coli strain from the Iberian wolf

  1. Gonçalves, A. 25
  2. Poeta, P. 25
  3. Monteiro, R. 25
  4. Marinho, C. 25
  5. Silva, N. 2
  6. Guerra, A. 4
  7. Petrucci-Fonseca, F. 4
  8. Rodrigues, J. 25
  9. Torres, C. 1
  10. Vitorino, R. 3
  11. Domingues, P. 3
  12. Igrejas, G. 5
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center for Animal Science and Veterinary, Vila Real, Portugal
  3. 3 Universidade de Aveiro
    info

    Universidade de Aveiro

    Aveiro, Portugal

    ROR https://ror.org/00nt41z93

  4. 4 Universidade de Lisboa
    info

    Universidade de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/01c27hj86

  5. 5 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Journal of Proteomics

ISSN: 1874-3919

Año de publicación: 2014

Volumen: 104

Páginas: 80-93

Tipo: Artículo

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DOI: 10.1016/J.JPROT.2014.02.033 SCOPUS: 2-s2.0-84901612363 WoS: WOS:000337878100008 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Proteomics

Repositorio institucional: lockAcceso abierto Editor

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2013/00001/001

Resumen

The Iberian wolf (Canis lupus signatus) is an endangered species native to the Iberian Peninsula. Due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. β-Lactam antibiotics like cefotaxime are the most commonly used antibacterial agents. Bacterial resistance to these antibiotics occurs predominantly through enzymatic inactivation by extended-spectrum beta-lactamases. Escherichia coli strain WA57, isolated from Iberian wolf feces, is a cefotaxime-resistant strain that produces extended-spectrum beta-lactamases. In this study, using 2D-GE combined with MS and bioinformatics, we report significant differences in the abundance of 40 protein spots (p < 0.01) from the extracellular, periplasmic, cytoplasmic, and membrane sub-proteomes and the whole-cell proteome of WA57 exposed and non-exposed to cefotaxime. A total of 315 protein spots were collected for protein identification. The comparative proteomics presented gives an overview of the complex changes in expression and metabolism that occur when WA57 is stressed with cefotaxime. Abundance of chaperone, porin and export proteins is particularly affected showing that the stress response and transport functions might directly influence the antibiotic resistance of this strain. Biological significance: This study highlights the importance of proteomics in detecting protein expression changes in bacterial strains exposed to stress such as that caused by cefotaxime. This approach might help us understand which pathways form barriers for antibiotics. This article is part of a Special Issue entitled: Environmental and structural proteomics. © 2014 Elsevier B.V. All rights reserved.